PYMEAcquire - Instrument control and simulation

PYMEAcquire is a general purpose microscope control and data acquisition platform with several features which make it well suited to localisation microscopy. These include:

• Support for commonly used hardware (eg Andor EMCCD cameras)

• Streaming to disk of large data series

• Acquisition protocols which allow various interactions with the hardware (eg controlling laser power) to occur at predetermined timepoints within an acquisition

• Tight integration with the real-time analysis tools

The basic usage should (hopefully) be relatively straight forward given some familiarity with other microscope control packages. Certain aspects, however deserve further description:

• Configuration
• Basic Usage
• Simple dSTORM/RPM walkthrough
• Simulator
• Acquisition Protocols
• Cameras / Detectors
• Stages / Translation
• Lasers / Light Sources
• Filters / Shutters
• Miscellaneous